Transcriptome and epigenome analysis of engram cells: Next-generation sequencing technologies in memory research

Transcription and epigenetic changes are integral components of the neuronal response to stimulation have been postulated be drivers or substrates for enduring in animal behavior, including learning memory. Memories thought deposited assemblies called engrams, i.e., groups cells that undergo persistent physical chemical during selectively reactivated retrieve Despite research progress made recent years, identity specific changes, if any, occur these subsequently contribute persistence memory traces remains unknown. The analysis is challenging due difficulty exploring molecular alterations only a relatively small percentage embedded complex tissue. In this review, we discuss advances field promise next-generation sequencing (NGS) epigenome editing methods overcoming challenges address long-standing questions concerning role mechanisms encoding, maintenance expression.